|Friday, 27 November, 2015, 08 : 00 AM [IST]|
|Ashwani Maindola, New Delhi|
The draft says provided further that steviol glycoside may be marketed as a table top sweetener in tablet form powder and liquid which may contain seven mg as steviol equivalent per 100 mg.
Steviol glycoside, according to the definition put up by FSSAI, is a white to light yellow powder, odourless or having a slight characteristic odour. About 200-300 times sweeter than sucrose. The product is obtained from the leaves of stevia rebaudiana bertoni. The leaves are extracted with hot water and the aqueous extract is passed through an adsorption resin to trap and concentrate the component steviol glycosides. The resin is washed with a solvent alcohol to release the glycosides and the product is recrystallised from methanol or aqueous ethanol. Ion exchange resins may be used in the purification process. The final product may be spray-dried. Stevioside and rebaudioside A are the component glycosides of principal interest for their sweetening property. Associated glycosides include rebaudioside B, rebaudioside C, rebaudioside D, rebaudioside F, dulcoside A, rubusoside and steviolbioside which are generally present in preparations of steviol glycosides at levels lower than stevioside or rebaudioside A.
Chemical name Stevioside:13-[(2-O-ß-D-glucopyranosyl-ß glucopyranosyl)oxy] kaur-16-en-18-oic acid, ß-D-glucopyranosyl ester
Rebaudioside A:13-[(2-O-ß-D-glucopyranosyl-3-O-ß-D-glucopyranosyl-ß-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, ß-D-glucopyranosyl ester
Freely soluble in water Stevioside and rebaudioside A
The main peak in the chromatogram obtained by following the procedure in Method of Assay corresponds to either stevioside or rebaudioside A
Not more than per cent
Not more than 200 mg/kg methanol and not more than 5000 mg/kg ethanol (Method I in Vol. 4, General Methods, Organic Components, Residual Solvents)
Not more than 1 mg/kg Determine by the atomic absorption hydride technique [Use Method II to prepare the test (sample) solution]
Not more than 1 mg/kg
Determine using an AAS/ICP-AES technique appropriate to the specified level. The selection of sample size and method of sample preparation may be based on the principles of the